BioPlayground
π§¬
Bio-Toolkit
gRNA Sequence Checker
CRISPR guide RNA μμ΄ κ²μ¦ λ° λΆμ
Score = f(GC%, poly-T, self-complementarity, PAM)
gRNA SEQUENCE
PAM TYPE
SpCas9 β NGG
SaCas9 β NNGRRT
Cas12a (Cpf1) β TTTV
Custom
RESTRICTION SITE CHECKS
BbsI
(GAAGAC)
β Golden Gate
BsaI
(GGTCTC)
β Golden Gate
BsmBI
(CGTCTC)
β Golden Gate (NEB)
EcoRI
(GAATTC)
β General cloning
BamHI
(GGATCC)
β General cloning
CUSTOM FORBIDDEN MOTIFS
Add
Copy
Reset